The genome sequence of an ichneumonid wasp, Amblyteles armatorius (Forster, 1771).

We present a genome assembly from an individual male Amblyteles armatorius (an ichneumonid wasp; Arthropoda; Insecta; Hymenoptera; Ichneumonidae). The genome sequence is 216 megabases in span. Most of the assembly is scaffolded into 12 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 16.6 kilobases in length.

The genome sequence of the Small Ranunculus, Hecatera dysodea (Denis & Schiffermüller, 1775).

We present a genome assembly from an individual female Hecatera dysodea (the Small Ranunculus; Arthropoda; Insecta; Lepidoptera; Noctuidae). The genome sequence is 640.9 megabases in span. Most of the assembly is scaffolded into 32 chromosomal pseudomolecules, including the Z and W sex chromosomes. The mitochondrial genome has also been assembled and is 15.4 kilobases in length. Gene annotation of this assembly on Ensembl has identified 12,213 protein coding genes.

The genome sequence of Clancy's Rustic, Caradrina kadenii (Freyer, 1836).

We present a genome assembly from an individual male Caradrina kadenii (Clancy's Rustic; Arthropoda; Insecta; Lepidoptera; Noctuidae). The genome sequence is 426.0 megabases in span. Most of the assembly is scaffolded into 31 chromosomal pseudomolecules, including the Z sex chromosome. The mitochondrial genome has also been assembled and is 15.4 kilobases in length.

A sampling strategy for genome sequencing the British terrestrial arthropod fauna.

The Darwin Tree of Life (DToL) project aims to sequence and assemble high-quality genomes from all eukaryote species in Britain and Ireland, with the first phase of the project concentrating on family-level coverage plus species of particular ecological, biomedical or evolutionary interest. We summarise the processes involved in (1) assessing the UK arthropod fauna and the status of individual species on UK lists; (2) prioritising and collecting species for initial genome sequencing; (3) handling methods to ensure that high-quality genomic DNA is preserved; and (4) compiling standard operating procedures for processing specimens for genome sequencing, identification verification and voucher specimen curation. We briefly explore some lessons learned from the pilot phase of DToL and the impact of the Covid-19 pandemic.

The genome sequence of the Grey Dagger, Acronicta psi (Linnaeus, 1758).

We present a genome assembly from an individual male Acronicta psi (the Grey Dagger; Arthropoda; Insecta; Lepidoptera; Noctuidae). The genome sequence is 405 megabases in span. The whole assembly is scaffolded into 31 chromosomal pseudomolecules, including the assembled Z sex chromosome. The mitochondrial genome has also been assembled and is 15.4 kilobases long.

The genome sequence of the Four-dotted Footman, Cybosia mesomella (Linnaeus, 1758).

We present a genome assembly from an individual male Cybosia mesomella (the Four-dotted Footman; Arthropoda; Insecta; Lepidoptera; Erebidae). The genome sequence is 948 megabases in span. Most of the assembly is scaffolded into 31 chromosomal pseudomolecules, with the Z sex chromosome assembled. The mitochondrial genome has also been assembled and is 15.4 kilobases in length.

The genome sequence of the ichneumon wasp Buathra laborator (Thunberg, 1822).

We present a genome assembly from an individual Buathra laborator (Arthropoda; Insecta; Hymenoptera; Ichneumonidae). The genome sequence is 330 megabases in span. Over 60% of the assembly is scaffolded into 11 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 35.8 kilobases in length.

The genome sequence of the Festoon, Apoda limacodes (Hufnagel, 1766).

We present a genome assembly from an individual male Apoda limacodes (the Festoon; Arthropoda; Insecta; Lepidoptera; Limacodidae). The genome sequence is 800 megabases in span. Most of the assembly is scaffolded into 25 chromosomal pseudomolecules, including the assembled Z sex chromosome. The mitochondrial genome has also been assembled and is 15.4 kilobases in length.

Signatures of increasing environmental stress in bumblebee wings over the past century: Insights from museum specimens.

Determining when animal populations have experienced stress in the past is fundamental to understanding how risk factors drive contemporary and future species' responses to environmental change. For insects, quantifying stress and associating it with environmental factors has been challenging due to a paucity of time-series data and because detectable population-level responses can show varying lag effects. One solution is to leverage historic entomological specimens to detect morphological proxies of stress experienced at the time stressors emerged, allowing us to more accurately determine population responses. Here we studied specimens of four bumblebee species, an invaluable group of insect pollinators, from five museums collected across Britain over the 20th century. We calculated the degree of fluctuating asymmetry (FA; random deviations from bilateral symmetry) between the right and left forewings as a potential proxy of developmental stress. We: (a) investigated whether baseline FA levels vary between species, and how this compares between the first and second half of the century; (b) determined the extent of FA change over the century in the four bumblebee species, and whether this followed a linear or nonlinear trend; (c) tested which annual climatic conditions correlated with increased FA in bumblebees. Species differed in their baseline FA, with FA being higher in the two species that have recently expanded their ranges in Britain. Overall, FA significantly increased over the century but followed a nonlinear trend, with the increase starting c. 1925. We found relatively warm and wet years were associated with higher FA. Collectively our findings show that FA in bumblebees increased over the 20th century and under weather conditions that will likely increase in frequency with climate change. By plotting FA trends and quantifying the contribution of annual climate conditions on past populations, we provide an important step towards improving our understanding of how environmental factors could impact future populations of wild beneficial insects.

The genome sequence of the Shuttle-shaped Dart, Agrotis puta (Hübner, 1803).

We present a genome assembly from an individual male Agrotis puta (the Shuttle-shaped Dart; Arthropoda; Insecta; Lepidoptera; Noctuidae). The genome sequence is 522 megabases in span. Most of the assembly is scaffolded into 31 chromosomal pseudomolecules, including the assembled Z chromosome. The mitochondrial genome has also been assembled and is 15.4 kilobases in length. Gene annotation of this assembly on Ensembl has identified 15,136 protein coding genes.

The genome sequence of an ichneumonid wasp, Heteropelma amictum (Fabricius, 1775).

We present a genome assembly from an individual female Heteropelma amictum (an ichneumonid wasp; Arthropoda; Insecta; Hymenoptera; Ichneumonidae). The genome sequence is 226.4 megabases in span. Most of the assembly is scaffolded into 10 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 20.65 kilobases in length.

The genome sequence of the Turnip Sawfly, Athalia rosae (Linnaeus, 1758).

We present a genome assembly from an individual female Athalia rosae (the Turnip Sawfly; Arhropoda; Insecta; Hymenoptera; Athaliidae). The genome sequence is 172 megabases in span. Most of the assembly is scaffolded into eight chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 16.3 kilobases in length. Gene annotation of this assembly on Ensembl identified 11,393 protein coding genes.

The genome sequence of the tree wasp, Dolichovespula sylvestris Scopoli, 1763.

We present a genome assembly from an individual male Dolichovespula sylvestris (the tree wasp; Arthropoda; Insecta; Hymenoptera; Vespidae). The genome sequence is 233 megabases in span. The majority of the assembly (95.56%) is scaffolded into 26 chromosomal pseudomolecules. The mitochondrial genome was also assembled and is 21.3 kilobases in length.

The genome sequence of a parasitoid wasp, Ichneumon xanthorius Forster, 1771.

We present a genome assembly from an individual female Ichneumon xanthorius (Arthropoda; Insecta; Hymenoptera; Ichneumonidae). The genome sequence is 315 megabases in span. The majority of the assembly (82.64%) is scaffolded into 12 chromosomal pseudomolecules. Gene annotation of this assembly on Ensembl has identified 10,622 protein coding genes.

The genome sequence of the setaceous Hebrew character, Xestia c-nigrum, (Linnaeus, 1758).

We present a genome assembly from an individual male Xestia c-nigrum (the setaceous Hebrew character; Arthropoda; Insecta; Lepidoptera; Noctuidae). The genome sequence is 760 megabases in span. Most of the assembly is scaffolded into 31 chromosomal pseudomolecules, including the assembled Z sex chromosome. The mitochondrial genome has also been assembled and is 15.3 kilobases in length.

The genome sequence of the dotted bee-fly, Bombylius discolor (Mikan, 1796).

We present a genome assembly from an individual female Bombylius discolor (the dotted bee-fly; Arthropoda; Insecta; Diptera; Bombyliidae). The genome sequence is 280 megabases in span. Most of the assembly (99.93%) is scaffolded into six chromosomal pseudomolecules, with the X sex chromosome assembled. The mitochondrial genome has also been assembled and is 16.7 kilobases in length. Genome annotation identified 10,411 protein-coding genes.

The genome sequence of Tenthredo notha Klug, 1814, a sawfly.

We present a genome assembly from an individual female Tenthredo notha (Arthropoda; Insecta; Hymenoptera; Tenthredinidae). The genome sequence is 253 megabases in span. Most of the assembly (99.91%) is scaffolded into 20 chromosomal pseudomolecules. The mitochondrial genome was also assembled and is 19.8 kilobases in length. Gene annotation of this assembly on Ensembl has identified 10,235 protein coding genes.

The tribe Rhaconotini (Hymenoptera: Braconidae: Doryctinae) of South Korea, with description of a new Rhaconotus Ruthe species.

The genera and species of the tribe Rhaconotini are reviewed for the fauna of the Korean Peninsula. A new Rhaconotus species, Rh. koreanus sp. nov., is described and illustrated. The generic placement of the Oriental Rhyssalus striatulus Cameron is corrected and Ipodoryctes species described from China, overlooked in a recent publication on Rhaconotini, are reclassified. The following are new combinations: Rhyssalus striatulus Cameron, 1909 = Mimipodoryctes striatulus (Cameron, 1909) comb. nov.; Ipodoryctes guizhouensis Tang et Chen, 2011 = Rhaconotinus (Rhaconotinus) guizhouensis (Tang et Chen, 2011), comb. nov.; Ipodoryctes hebeiensis Tang et Chen, 2011 = Rhaconotinus (Rhaconotinus) hebeiensis (Tang et Chen, 2011), comb. nov.; Ipodoryctes rugosus Tang et Chen, 2011 = Rhaconotinus (Rhaconotinus) rugosus (Tang et Chen, 2011), comb. nov.; Ipodoryctes rutilans Tang et Chen, 2011 = Rhaconotinus (Rhaconotinus) rutilans (Tang et Chen, 2011), comb. nov.; Ipodoryctes wuyiensis Tang et Chen, 2011 = Rhaconotinus (Rhaconotinus) wuyiensis (Tang et Chen, 2011), comb. nov.; and Ipodoryctes liui Tang et Chen, 2011 = Rhaconotinus (Hexarhaconotinus) liui (Tang et Chen, 2011), comb. nov.

Taxonomic changes in Ichneumonoidea (Hymenoptera), and notes on certain type specimens.

The following new synonymies are established: Acrodactyla iliensis Sheng Bian 1996 = Acrodactyla lachryma Pham, Broad, Matsumoto Böhme 2012, syn. nov.; Euceros Gravenhorst 1829 = Lentocerus Dong Naito 1999, syn. nov.; Euceros pruinosus (Gravenhorst 1829) = Lentocerus dentatus Dong Naito 1999, syn. nov.; Euceros sensibus Uchida 1930 = Lentocerus lijiangensis Dong Naito 1999, syn. nov.; Gyroneuron Kokujev 1901 = Cyclophatnus Cameron 1910, syn. nov.; Gyroneuron flavum (Cameron 1910) = Gyroneuron testaceator Watanabe 1934, syn. nov.; Liotryphon strobilellae (Linnaeus 1758) = Townesia qinghaiensis He 1996, syn. nov. The following are new combinations: Aleiodes insignis (Brues 1926), Aleiodes lateralis (Cameron 1905), Aleiodes maculicornis (Brues 1926), Aleiodes siccitesta (Morley 1937), Cyclophatnus flavum (Cameron 1910), Rhaconotus striatulus (Cameron 1909), Tolonus cingulatorius (Morley 1912), Zatypota tropica (Morley 1912). Netelia morleyi Townes, Townes Gupta 1961 is transferred from the subgenus Netelia Gray 1860 to the subgenus Paropheltes Cameron 1907. One new replacement name is proposed: Aleiodes philippinensis nom. nov. for Rhogas lateralis Baker 1917, nec Troporhogas lateralis Cameron 1905. Lectotypes are designated for Antrusa persimilis Nixon 1954, Rhyssalus striatulus Cameron 1909, Troporhogas trimaculata Cameron 1905, Hemiteles cingulatorius Morley 1912, Paniscus ferrugineus Cameron 1889 and for Xanthojoppa inermis Morley 1917. Some previously overlooked type specimens are interpreted and illustrated and some errors in the literature corrected. Hosts are recorded for two genera of Ichneumoninae for the first time: Catadelphops nasutus (Heinrich 1962) was reared from Proserpinus terlooii (Edwards 1875) (Lepidoptera: Sphingidae) in the USA, and Aethianoplis excavata (Roman 1910) was reared from Precis octavia (Cramer 1777) (Lepidoptera: Nymphalidae) in Uganda.

Phylogenomics of Ichneumonoidea (Hymenoptera) and implications for evolution of mode of parasitism and viral endogenization.

Ichneumonoidea is one of the most diverse lineages of animals on the planet with >48,000 described species and many more undescribed. Parasitoid wasps of this superfamily are mostly beneficial insects that attack and kill other arthropods and are important for understanding diversification and the evolution of life history strategies related to parasitoidism. Further, some lineages of parasitoids within Ichneumonoidea have acquired endogenous virus elements (EVEs) that are permanently a part of the wasp's genome and benefit the wasp through host immune disruption and behavioral control. Unfortunately, understanding the evolution of viral acquisition, parasitism strategies, diversification, and host immune disruption mechanisms, is deeply limited by the lack of a robust phylogenetic framework for Ichneumonoidea. Here we design probes targeting 541 genes across 91 taxa to test phylogenetic relationships, the evolution of parasitoid strategies, and the utility of probes to capture polydnavirus genes across a diverse array of taxa. Phylogenetic relationships among Ichneumonoidea were largely well resolved with most higher-level relationships maximally supported. We noted codon use biases between the outgroups, Braconidae, and Ichneumonidae and within Pimplinae, which were largely solved through analyses of amino acids rather than nucleotide data. These biases may impact phylogenetic reconstruction and caution for outgroup selection is recommended. Ancestral state reconstructions were variable for Braconidae across analyses, but consistent for reconstruction of idiobiosis/koinobiosis in Ichneumonidae. The data suggest many transitions between parasitoid life history traits across the whole superfamily. The two subfamilies within Ichneumonidae that have polydnaviruses are supported as distantly related, providing strong evidence for two independent acquisitions of ichnoviruses. Polydnavirus capture using our designed probes was only partially successful and suggests that more targeted approaches would be needed for this strategy to be effective for surveying taxa for these viral genes. In total, these data provide a robust framework for the evolution of Ichneumonoidea.